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lectins  (Vector Laboratories)


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    Structured Review

    Vector Laboratories lectins
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1170-2/Unconjugated+Lycopersicon+Esculentum+(Tomato)+Lectin+(LEL%2C+TL)/pm41691071-214-0-3
    Average 94 stars, based on 670 article reviews
    lectins - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: A multiscale vascular atlas of blood vessels supplying the urinary bladder of male and female mice
    Article Snippet: Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) , AF594 , , Vector Laboratories , DL-1177–1 , , N/A.

    Article Title: Endothelial and neuronal engagement by AAV-BR1 gene therapy alleviates neurological symptoms and lipid deposition in a mouse model of Niemann-Pick type C2.
    Article Snippet: Sections double stained for NeuN and/or NPC2 were visualized using goat anti-mouse Alexa 594 (Invitrogen #A11032) (1:200), and biotinylated goat antirabbit (1:200).

    Article Title: Endothelial and neuronal engagement by AAV-BR1 gene therapy alleviates neurological symptoms and lipid deposition in a mouse model of Niemann-Pick type C2
    Article Snippet: The sections were again washed in PPBS and visualized using an anti-streptavidin Alexa 488 (Invitrogen, #S32354) (1:200) antibody for one hour.

    Labeling:

    Article Title: Visualization of porcine and human aqueous humor outflow tract anatomies with transparency enhancement.
    Article Snippet: and surrounding tissues of the retina and choroid have been observed in vivo using fluorescein angiography, indocyanine green angiography, optical coherence tomography, and optical coherence tomography angiography, which have led to new pathological findings [2, 3].. However, current in vivo observations are inferior to tissue microscopy in terms of resolution and evaluation of deep structures.. Moreover, the relationship to surrounding tissues is challenging in the assessment of injection-molded specimens.

    Saline:

    Article Title: Visualization of porcine and human aqueous humor outflow tract anatomies with transparency enhancement.
    Article Snippet: and surrounding tissues of the retina and choroid have been observed in vivo using fluorescein angiography, indocyanine green angiography, optical coherence tomography, and optical coherence tomography angiography, which have led to new pathological findings [2, 3].. However, current in vivo observations are inferior to tissue microscopy in terms of resolution and evaluation of deep structures.. Moreover, the relationship to surrounding tissues is challenging in the assessment of injection-molded specimens.

    Incubation:

    Article Title: Vascular extracellular matrix hydrogel
    Article Snippet: After 72 hr on the CAM, bright field images of the scaffolds and surrounding vasculature resulting from the angiogenic response were captured using a 3MP color camera mounted on a stereomicroscope (AmScope, Irvine, Calif.) at a 7.5× magnification. .. Endothelial cells of the chick vasculature were labelled by micro-injecting DyLight® 650-labeled tomato lectin (Vector labs, Burlingame, Calif.) and incubated for 15 min prior to excising the scaffold and the surrounding CAM. .. The harvested tissue was fixed in 10% neutral-buffered formalin (Sigma, St. Louis, Mo.) for 48 hr, washed in 1×PBS thrice and cryoprotected for 72 hr in 30% sucrose solution before processing for histological evaluation.

    Chick Chorioallantoic Membrane Assay:

    Article Title: Vascular extracellular matrix hydrogel
    Article Snippet: After 72 hr on the CAM, bright field images of the scaffolds and surrounding vasculature resulting from the angiogenic response were captured using a 3MP color camera mounted on a stereomicroscope (AmScope, Irvine, Calif.) at a 7.5× magnification. .. Endothelial cells of the chick vasculature were labelled by micro-injecting DyLight® 650-labeled tomato lectin (Vector labs, Burlingame, Calif.) and incubated for 15 min prior to excising the scaffold and the surrounding CAM. .. The harvested tissue was fixed in 10% neutral-buffered formalin (Sigma, St. Louis, Mo.) for 48 hr, washed in 1×PBS thrice and cryoprotected for 72 hr in 30% sucrose solution before processing for histological evaluation.



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    Screening of lectins for detection of native glycoRNAs. ( a ) Twenty-four lectins were purchased from Vector Laboratories and screened for their ability to detect native glycoRNAs. Total RNA (10 µg) isolated from THP-1 cells was used for lectin screening. As a positive control, input RNA (10 µg) isolated from Ac₄ManNAz-labeled THP-1 cells was conjugated with biotin and detected using anti-biotin-HRP. All lectins were used at a concentration of 1.5 µg/mL. Experiments were repeated twice. ( b ) Band intensities were quantified using iBright internal software. Information on the primary sugar specificity of lectins was obtained from Vector Laboratories.

    Journal: Scientific Reports

    Article Title: Lectin-based detection and expression profiling of native glycoRNAs

    doi: 10.1038/s41598-026-40291-2

    Figure Lengend Snippet: Screening of lectins for detection of native glycoRNAs. ( a ) Twenty-four lectins were purchased from Vector Laboratories and screened for their ability to detect native glycoRNAs. Total RNA (10 µg) isolated from THP-1 cells was used for lectin screening. As a positive control, input RNA (10 µg) isolated from Ac₄ManNAz-labeled THP-1 cells was conjugated with biotin and detected using anti-biotin-HRP. All lectins were used at a concentration of 1.5 µg/mL. Experiments were repeated twice. ( b ) Band intensities were quantified using iBright internal software. Information on the primary sugar specificity of lectins was obtained from Vector Laboratories.

    Article Snippet: Lectins (including LEL, Vector Lab, L-1170-2) and Ac4ManNAz were purchased from Vector Laboratories.

    Techniques: Plasmid Preparation, Isolation, Positive Control, Labeling, Concentration Assay, Software

    Sensitivity and specificity of lectin-based detection of native glycoRNAs. ( a ) Sensitivity comparison of the three indicated detection methods. Increasing amounts of total RNA isolated from Ac₄ManNAz-labeled or unlabeled THP-1 cells were analyzed. ( b ) Total RNA (10 µg) from THP-1 cells treated with or without NGI-1 (4 µM) or kifunensine (Kif; 1 µM) was analyzed by lectin-based detection (LBD) using LEL (3 µg/mL). ( c ) Total RNA (10 µg) from THP-1 cells was treated with RNase cocktail (1 µL; RNase A, 0.5 U/µL and RNase T1, 20 U/µL), DNase I (1 U/µL), PNGase F (1 U/µL), or Proteinase K (2 µg/µL) at 37 °C for 10 min. After purification using Zymo columns, samples were analyzed by LBD with LEL (3 µg/mL). ( d ) Total RNA (10 µg) from mouse colon tissue was treated with RNase cocktail, DNase I (1 U/µL), or Proteinase K (2 µg/µL) at 37 °C for 10 min. After purification, RNA samples were analyzed by LBD using LEL (3 µg/mL). All experiments were repeated twice.

    Journal: Scientific Reports

    Article Title: Lectin-based detection and expression profiling of native glycoRNAs

    doi: 10.1038/s41598-026-40291-2

    Figure Lengend Snippet: Sensitivity and specificity of lectin-based detection of native glycoRNAs. ( a ) Sensitivity comparison of the three indicated detection methods. Increasing amounts of total RNA isolated from Ac₄ManNAz-labeled or unlabeled THP-1 cells were analyzed. ( b ) Total RNA (10 µg) from THP-1 cells treated with or without NGI-1 (4 µM) or kifunensine (Kif; 1 µM) was analyzed by lectin-based detection (LBD) using LEL (3 µg/mL). ( c ) Total RNA (10 µg) from THP-1 cells was treated with RNase cocktail (1 µL; RNase A, 0.5 U/µL and RNase T1, 20 U/µL), DNase I (1 U/µL), PNGase F (1 U/µL), or Proteinase K (2 µg/µL) at 37 °C for 10 min. After purification using Zymo columns, samples were analyzed by LBD with LEL (3 µg/mL). ( d ) Total RNA (10 µg) from mouse colon tissue was treated with RNase cocktail, DNase I (1 U/µL), or Proteinase K (2 µg/µL) at 37 °C for 10 min. After purification, RNA samples were analyzed by LBD using LEL (3 µg/mL). All experiments were repeated twice.

    Article Snippet: Lectins (including LEL, Vector Lab, L-1170-2) and Ac4ManNAz were purchased from Vector Laboratories.

    Techniques: Comparison, Isolation, Labeling, Purification